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Revista de Chimie (Rev. Chim.), Year 2020, Volume 71, Issue 7, 481-490

https://doi.org/10.37358/RC.20.7.8267

Bogdan Sorop, Vlad Laurentiu David, Alina Heghes, Delia Berceanu-Vaduva, Lavinia Balan, Maria Sorop Florea, Doru Anastasiu

Folates and Anti-folates - Chemical Perspectives, in vitro and in ovo Assessments

Abstract:

Folates participate in DNA replication reactions, act as a substrate in enzymatic reactions related to amino acid synthesis and vitamin metabolism while antifolates participate in reactions that inhibit the formation of tetrahydrofolate with consequences on protein and nucleic acid synthesis and implicitly on growth and development both types of cells, healthy and diseased. In the present study, the viability of healthy cells, keratinocytes and human fibroblasts was evaluated in the presence of three folates (folic, dihydrofolic and tetrahydrofolic acids), one antifolate (methotrexate) and combinations between them by Alamar blue assay. The antiangiogenic potential was also evaluated by in ovo technique, CAM assay. Cell viability was influenced in a cell-dependent and dose-dependent manner, fibroblasts being more sensitive to the action of the test compounds, especially the combination of metrotrexate and dihydrofolate. Data related to CAM assay showed that methotrexate revealed a slightly higher vessel density, but without inducing toxicity on vascular architecture and functionality. The data obtained highlight the greater sensitivity of the viability of fibroblasts in the presence of metrotrexate and its combinations with folates used in the study.
Keywords:
folates; methotrexate; reactivity; viability; irritation

Issue: 2020, Volume 71, Issue 7
Pages: 481-490
Publication date: 2020/8/4
https://doi.org/10.37358/RC.20.7.8267
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Creative Commons License
This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
SOROP, B., DAVID, V.L., HEGHES, A., BERCEANU-VADUVA, D., BALAN, L., FLOREA, M.S., ANASTASIU, D., Folates and Anti-folates - Chemical Perspectives, in vitro and in ovo Assessments, Rev. Chim., 71(7), 2020, 481-490.

Vancouver
Sorop B, David VL, Heghes A, Berceanu-Vaduva D, Balan L, Florea MS, Anastasiu D. Folates and Anti-folates - Chemical Perspectives, in vitro and in ovo Assessments. Rev. Chim.[internet]. 2020 Jul;71(7):481-490. Available from: https://doi.org/10.37358/RC.20.7.8267


APA 6th edition
Sorop, B., David, V.L., Heghes, A., Berceanu-Vaduva, D., Balan, L., Florea, M.S. & Anastasiu, D. (2020). Folates and Anti-folates - Chemical Perspectives, in vitro and in ovo Assessments. Revista de Chimie, 71(7), 481-490. https://doi.org/10.37358/RC.20.7.8267


Harvard
Sorop, B., David, V.L., Heghes, A., Berceanu-Vaduva, D., Balan, L., Florea, M.S., Anastasiu, D. (2020). 'Folates and Anti-folates - Chemical Perspectives, in vitro and in ovo Assessments', Revista de Chimie, 71(7), pp. 481-490. https://doi.org/10.37358/RC.20.7.8267


IEEE
B. Sorop, V.L. David, A. Heghes, D. Berceanu-Vaduva, L. Balan, M.S. Florea, D. Anastasiu, "Folates and Anti-folates - Chemical Perspectives, in vitro and in ovo Assessments". Revista de Chimie, vol. 71, no. 7, pp. 481-490, 2020. [online]. https://doi.org/10.37358/RC.20.7.8267


Text
Bogdan Sorop, Vlad Laurentiu David, Alina Heghes, Delia Berceanu-Vaduva, Lavinia Balan, Maria Sorop Florea, Doru Anastasiu,
Folates and Anti-folates - Chemical Perspectives, in vitro and in ovo Assessments,
Revista de Chimie,
Volume 71, Issue 7,
2020,
Pages 481-490,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.7.8267.
(https://revistadechimie.ro/Articles.asp?ID=8267)
Keywords: folates; methotrexate; reactivity; viability; irritation


RIS
TY - JOUR
T1 - Folates and Anti-folates - Chemical Perspectives, in vitro and in ovo Assessments
A1 - Sorop, Bogdan
A2 - David, Vlad Laurentiu
A3 - Heghes, Alina
A4 - Berceanu-Vaduva, Delia
A5 - Balan, Lavinia
A6 - Florea, Maria Sorop
A7 - Anastasiu, Doru
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 7
SP - 481
EP - 490
UR - https://doi.org/10.37358/RC.20.7.8267
KW - folates
KW - methotrexate
KW - reactivity
KW - viability
KW - irritation
ER -


BibTex
@article{RevCh2020P481,
author = {Sorop Bogdan and David Vlad Laurentiu and Heghes Alina and Berceanu-Vaduva Delia and Balan Lavinia and Florea Maria Sorop and Anastasiu Doru},
title = {Folates and Anti-folates - Chemical Perspectives, in vitro and in ovo Assessments},
journal = {Revista de Chimie},
volume = {71},
number = {7},
pages = {481-490},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.7.8267},
url = {https://revistadechimie.ro/Articles.asp?ID=8267}
}
 
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