Determination of Scopolamine by Gas Chromatography from Different Parts of the Datura innoxia Biomass

Mardare (Balusescu), Georgiana; Malutan, Teodor; Lazar, Liliana

Georgiana Mardare (Balusescu), Teodor Malutan, Liliana Lazar

Determination of Scopolamine by Gas Chromatography from Different Parts of the Datura innoxia Biomass

Abstract:

Tropane alkaloids are characteristic secondary metabolites which occurred mainly in Solanaceae plant family. Among them, hyoscine or scopolamine is one of the predominant in the Datura species, occurring in all plant organs (vegetative and reproductive). Because of its medical use and toxic properties, a rapid, convenient and reliable method is essential for the quantification of scopolamine content of plant biomass. The aim of this study was to elaborate a rapid method for determination of scopolamine from Datura innoxia, using Soxhlet extraction and quantification by gas-cromatography with flame ionization detection. Scopolamine content was investigated in plant organs (leaves, flowers, fruits, stem end root) of Datura innoxia collected in the maturity period from a country side region from Romania. Scopolamine was found in all organs examined. The results revealed that, concentration of scopolamine extracted in ethanol followed the order: root ] seeds ] flowers ] stem ] leaves. Higher extraction efficiency of scopolamine was found for 1-butanol compared to ethanol, and the order of concentration was: flowers ] seeds ] leaves ] stem ] root. Scopolamine content showed differences indicating that it is influenced by: 1) plant biomass as place of growing, plant species, plant organ and 2) extraction process efficiency based on polarity of the solvent, extraction time and sample - solvent ratio. The results show clear evidence that, the highest scopolamine content was found in 1-butanolic extracts and lowest in ethanolic extracts.

Keywords:
GC-FID; scopolamine; tropane alkaloids; Soxhlet extraction; ethanol; 1-butanol

Issue: 2020, Volume 71, Issue 7

Pages: 126-136

Publication date: 2020/8/4

https://doi.org/10.37358/RC.20.7.8230

Download Pdf Article

This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
MARDARE (BALUSESCU), G., MALUTAN, T., LAZAR, L., Determination of Scopolamine by Gas Chromatography from Different Parts of the Datura innoxia Biomass, Rev. Chim., 71(7), 2020, 126-136.

Vancouver
Mardare (Balusescu) G, Malutan T, Lazar L. Determination of Scopolamine by Gas Chromatography from Different Parts of the Datura innoxia Biomass. Rev. Chim.[internet]. 2020 Jul;71(7):126-136. Available from: https://doi.org/10.37358/RC.20.7.8230

APA 6th edition
Mardare (Balusescu), G., Malutan, T. & Lazar, L. (2020). Determination of Scopolamine by Gas Chromatography from Different Parts of the Datura innoxia Biomass. Revista de Chimie, 71(7), 126-136. https://doi.org/10.37358/RC.20.7.8230

Harvard
Mardare (Balusescu), G., Malutan, T., Lazar, L. (2020). 'Determination of Scopolamine by Gas Chromatography from Different Parts of the Datura innoxia Biomass', Revista de Chimie, 71(7), pp. 126-136. https://doi.org/10.37358/RC.20.7.8230

IEEE
G. Mardare(Balusescu), T. Malutan, L. Lazar, "Determination of Scopolamine by Gas Chromatography from Different Parts of the Datura innoxia Biomass". Revista de Chimie, vol. 71, no. 7, pp. 126-136, 2020. [online]. https://doi.org/10.37358/RC.20.7.8230

Text
Georgiana Mardare(Balusescu), Teodor Malutan, Liliana Lazar,
Determination of Scopolamine by Gas Chromatography from Different Parts of the Datura innoxia Biomass,
Revista de Chimie,
Volume 71, Issue 7,
2020,
Pages 126-136,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.7.8230.
(https://revistadechimie.ro/Articles.asp?ID=8230)
Keywords: GC-FID; scopolamine; tropane alkaloids; Soxhlet extraction; ethanol; 1-butanol

RIS
TY - JOUR
T1 - Determination of Scopolamine by Gas Chromatography from Different Parts of the Datura innoxia Biomass
A1 - Mardare (Balusescu), Georgiana
A2 - Malutan, Teodor
A3 - Lazar, Liliana
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 7
SP - 126
EP - 136
UR - https://doi.org/10.37358/RC.20.7.8230
KW - GC-FID
KW - scopolamine
KW - tropane alkaloids
KW - Soxhlet extraction
KW - ethanol
KW - 1-butanol
ER -

BibTex
@article{RevCh2020P126,
author = {Mardare (Balusescu) Georgiana and Malutan Teodor and Lazar Liliana},
title = {Determination of Scopolamine by Gas Chromatography from Different Parts of the Datura innoxia Biomass},
journal = {Revista de Chimie},
volume = {71},
number = {7},
pages = {126-136},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.7.8230},
url = {https://revistadechimie.ro/Articles.asp?ID=8230}
}

<<<< back

PUBLISHING SECTION

Author Guidelines

* Paper Drafting

* Paper Template

* Ethical Statement

* Paper Submission

Ghidul Autorului (ro)

Open Access & Archiving Policy

Search Authors

LATEST NEWS
	NUMAR OMAGIAL PETRU PONI.. detalii
	Revista de Chimie - 70 de ani de aparitie neintrerupta.. detalii
	English-Romanian Dictionary for Mechanical Engineering (download pdf)