Neuroprotective Effects of Low Intensity and Low Frequency Electromagnetic ”in vitro” Stimulation on Glial Cells

Dascalu, Ana Maria; Serban, Dragos; Vladila, Bogdan; Grigore, Alice; Socea, Bogdan; Sabau, Alexandru; Tudosie, Mihail Silviu; Badiu, Cristinel Dumitru; Smarandache, Gabriel Catalin

Ana Maria Dascalu, Dragos Serban, Bogdan Vladila, Alice Grigore, Bogdan Socea, Alexandru Sabau, Mihail Silviu Tudosie, Cristinel Dumitru Badiu, Gabriel Catalin Smarandache

Neuroprotective Effects of Low Intensity and Low Frequency Electromagnetic ”in vitro” Stimulation on Glial Cells

Abstract:

Degenerative neurological diseases (senile dementia, Alzheimer's disease, glaucoma), post-stroke sequelae are increasing as prevalence nowadays, in context of aging in general population, and there are strong evidences that astrocytes may play a significant role in neuroprotection. The study evaluates the effects of low intensity low frequency electromagnetic field (EMF) „in vitro” stimulation (195 mA, 7-8 Hz) on the glial cells, in different conditions, in order to identify the neuroprotective potential. Three cell lines were used for the study: the Clonetics (Lonza) line of normal human astrocytes, U87 glioblastoma tumor cells (ATCC) and L929 murine fibroblasts. The cell cultures were exposed to EMF stimulation for 2 hours/day, for 5 and 10 day, in absence and in the presence of oxidative stress stimuli. Cell viability, apoptotic potential, changes in cell phenotype, stimulation of cell proliferation for normal and malignant transformed cell lines, resilience to oxidative stress were investigated. The viability of the cells was investigated by MTS assay, and the Griess test was used to detect nitrite production as a marker of oxidation and inflammation. Inverted microscopy examination revealed that following exposure to the tested EMF, no morphological changes of the cells were recorded. In none of the cell lines tested, no cytotoxic or cytopathic effects were observed in the cultures exposed under low density conditions. In case of exposure of cell cultures for 10 days to the action of EMF, a viability of 98.2% of glial cells and 97% of exposed fibroblasts is observed, compared to unexposed cells. The viability of the cells exposed to both EMF and oxidative and inflammatory stimuli is increased by 0.96% in the case of H2O2 stimulation and in 3.76% in the case of LPS stimulation, suggesting a possible neuroprotective effect of EMF exposure. Low intensity low frequency EMF ”in vitro” stimulation increased viability of the glial cells exposed to oxidative and inflammatory stimuli. No cytotoxic or cytopathic effects were observed in the exposed cultures under conditions of low density. Further tests are needed in order to elucidate the mechanisms of action of EMF on astrocytes, especially on astrocyte-neuron co-cultures to highlight the interactions between these cell types. Confirmation of the neuroprotective effect of low intensity and low frequency EMF opens the way for the development of an innovative, non-invasive therapy.

Keywords:
astrocytes; electromagnetic field; neuroprotection

Issue: 2020, Volume 71, Issue 2

Pages: 330-334

Publication date: 2020/3/3

https://doi.org/10.37358/RC.20.2.7933

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
DASCALU, A.M., SERBAN, D., VLADILA, B., GRIGORE, A., SOCEA, B., SABAU, A., TUDOSIE, M.S., BADIU, C.D., SMARANDACHE, G.C., Neuroprotective Effects of Low Intensity and Low Frequency Electromagnetic ”in vitro” Stimulation on Glial Cells, Rev. Chim., 71(2), 2020, 330-334.

Vancouver
Dascalu AM, Serban D, Vladila B, Grigore A, Socea B, Sabau A, Tudosie MS, Badiu CD, Smarandache GC. Neuroprotective Effects of Low Intensity and Low Frequency Electromagnetic ”in vitro” Stimulation on Glial Cells. Rev. Chim.[internet]. 2020 Feb;71(2):330-334. Available from: https://doi.org/10.37358/RC.20.2.7933

APA 6th edition
Dascalu, A.M., Serban, D., Vladila, B., Grigore, A., Socea, B., Sabau, A.,... Smarandache, G.C. (2020). Neuroprotective Effects of Low Intensity and Low Frequency Electromagnetic ”in vitro” Stimulation on Glial Cells. Revista de Chimie, 71(2), 330-334. https://doi.org/10.37358/RC.20.2.7933

Harvard
Dascalu, A.M., Serban, D., Vladila, B., Grigore, A., Socea, B., Sabau, A., Tudosie, M.S., Badiu, C.D., Smarandache, G.C. (2020). 'Neuroprotective Effects of Low Intensity and Low Frequency Electromagnetic ”in vitro” Stimulation on Glial Cells', Revista de Chimie, 71(2), pp. 330-334. https://doi.org/10.37358/RC.20.2.7933

IEEE
A.M. Dascalu, D. Serban, B. Vladila, A. Grigore, B. Socea, A. Sabau, M.S. Tudosie, C.D. Badiu, G.C. Smarandache, "Neuroprotective Effects of Low Intensity and Low Frequency Electromagnetic ”in vitro” Stimulation on Glial Cells". Revista de Chimie, vol. 71, no. 2, pp. 330-334, 2020. [online]. https://doi.org/10.37358/RC.20.2.7933

Text
Ana Maria Dascalu, Dragos Serban, Bogdan Vladila, Alice Grigore, Bogdan Socea, Alexandru Sabau, Mihail Silviu Tudosie, Cristinel Dumitru Badiu, Gabriel Catalin Smarandache,
Neuroprotective Effects of Low Intensity and Low Frequency Electromagnetic ”in vitro” Stimulation on Glial Cells,
Revista de Chimie,
Volume 71, Issue 2,
2020,
Pages 330-334,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.2.7933.
(https://revistadechimie.ro/Articles.asp?ID=7933)
Keywords: astrocytes; electromagnetic field; neuroprotection

RIS
TY - JOUR
T1 - Neuroprotective Effects of Low Intensity and Low Frequency Electromagnetic ”in vitro” Stimulation on Glial Cells
A1 - Dascalu, Ana Maria
A2 - Serban, Dragos
A3 - Vladila, Bogdan
A4 - Grigore, Alice
A5 - Socea, Bogdan
A6 - Sabau, Alexandru
A7 - Tudosie, Mihail Silviu
A8 - Badiu, Cristinel Dumitru
A9 - Smarandache, Gabriel Catalin
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 2
SP - 330
EP - 334
UR - https://doi.org/10.37358/RC.20.2.7933
KW - astrocytes
KW - electromagnetic field
KW - neuroprotection
ER -

BibTex
@article{RevCh2020P330,
author = {Dascalu Ana Maria and Serban Dragos and Vladila Bogdan and Grigore Alice and Socea Bogdan and Sabau Alexandru and Tudosie Mihail Silviu and Badiu Cristinel Dumitru and Smarandache Gabriel Catalin},
title = {Neuroprotective Effects of Low Intensity and Low Frequency Electromagnetic ”in vitro” Stimulation on Glial Cells},
journal = {Revista de Chimie},
volume = {71},
number = {2},
pages = {330-334},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.2.7933},
url = {https://revistadechimie.ro/Articles.asp?ID=7933}
}

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