Binding Affinities Studies of Nitrated Model Peptides to Monoclonal Anti-3-nitrotyrosine Antibody

Ion, Laura; Lupaescu, Ancuta-Veronica; Neamtu, Andrei; Drochioiu, Gabi; Petre, Brindusa-Alina

Laura Ion, Ancuta-Veronica Lupaescu, Andrei Neamtu, Gabi Drochioiu, Brindusa-Alina Petre

Binding Affinities Studies of Nitrated Model Peptides to Monoclonal Anti-3-nitrotyrosine Antibody

Abstract:

Tyrosine nitration has been shown to be an important oxidative protein modification and play a crucial role in pathophysiological conditions, associated with oxidative stress, such as atherosclerosis and neurodegenerative disease. For a better understanding of nitration mechanism, the identification and the quantification of nitration sites represents an important research goal. Due to (i) the low levels of nitration in native proteins, (ii) structural changes induced by nitration and (iii) the specificity of anti - 3 nitro tyrosine antibodies the method which may provide the identification of nitration sites in proteins represent a challenging experimental task. In this work we have used synthetic nitrated tyrosine containing peptides to determine antibody-binding affinities and specificity of different tyrosine residue (Tyr33, Tyr98, Tyr107and Tyr122) in Eosinophil cationic proteins (ECP). The highest affinity of nitrated ECP peptides to the monoclonal antibody (anti-3NT) was obtained for the only in vivo nitrated identified residue Tyr33(0.082 µM); in contrast, all other three nitrated residues at Tyr98, Tyr107 and Tyr122) showed significant lower affinity being imbedded in the ECP protein structure as similar resulted by molecular computational modeling.

Keywords:
proteintyrosine nitration; nitro-tyrosine antibodies; ELISA; circular dichroism spectroscopy

Issue: 2020, Volume 71, Issue 1

Pages: 259-265

Publication date: 2020/2/7

https://doi.org/10.37358/RC.20.1.7843

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
ION, L., LUPAESCU, A., NEAMTU, A., DROCHIOIU, G., PETRE, B., Binding Affinities Studies of Nitrated Model Peptides to Monoclonal Anti-3-nitrotyrosine Antibody, Rev. Chim., 71(1), 2020, 259-265.

Vancouver
Ion L, Lupaescu A, Neamtu A, Drochioiu G, Petre B. Binding Affinities Studies of Nitrated Model Peptides to Monoclonal Anti-3-nitrotyrosine Antibody. Rev. Chim.[internet]. 2020 Jan;71(1):259-265. Available from: https://doi.org/10.37358/RC.20.1.7843

APA 6th edition
Ion, L., Lupaescu, A., Neamtu, A., Drochioiu, G. & Petre, B. (2020). Binding Affinities Studies of Nitrated Model Peptides to Monoclonal Anti-3-nitrotyrosine Antibody. Revista de Chimie, 71(1), 259-265. https://doi.org/10.37358/RC.20.1.7843

Harvard
Ion, L., Lupaescu, A., Neamtu, A., Drochioiu, G., Petre, B. (2020). 'Binding Affinities Studies of Nitrated Model Peptides to Monoclonal Anti-3-nitrotyrosine Antibody', Revista de Chimie, 71(1), pp. 259-265. https://doi.org/10.37358/RC.20.1.7843

IEEE
L. Ion, A. Lupaescu, A. Neamtu, G. Drochioiu, B. Petre, "Binding Affinities Studies of Nitrated Model Peptides to Monoclonal Anti-3-nitrotyrosine Antibody". Revista de Chimie, vol. 71, no. 1, pp. 259-265, 2020. [online]. https://doi.org/10.37358/RC.20.1.7843

Text
Laura Ion, Ancuta-Veronica Lupaescu, Andrei Neamtu, Gabi Drochioiu, Brindusa-Alina Petre,
Binding Affinities Studies of Nitrated Model Peptides to Monoclonal Anti-3-nitrotyrosine Antibody,
Revista de Chimie,
Volume 71, Issue 1,
2020,
Pages 259-265,
ISSN 2668-8212,
https://doi.org/10.37358/RC.20.1.7843.
(https://revistadechimie.ro/Articles.asp?ID=7843)
Keywords: proteintyrosine nitration; nitro-tyrosine antibodies; ELISA; circular dichroism spectroscopy

RIS
TY - JOUR
T1 - Binding Affinities Studies of Nitrated Model Peptides to Monoclonal Anti-3-nitrotyrosine Antibody
A1 - Ion, Laura
A2 - Lupaescu, Ancuta-Veronica
A3 - Neamtu, Andrei
A4 - Drochioiu, Gabi
A5 - Petre, Brindusa-Alina
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2020
VL - 71
IS - 1
SP - 259
EP - 265
UR - https://doi.org/10.37358/RC.20.1.7843
KW - proteintyrosine nitration
KW - nitro-tyrosine antibodies
KW - ELISA
KW - circular dichroism spectroscopy
ER -

BibTex
@article{RevCh2020P259,
author = {Ion Laura and Lupaescu Ancuta-Veronica and Neamtu Andrei and Drochioiu Gabi and Petre Brindusa-Alina},
title = {Binding Affinities Studies of Nitrated Model Peptides to Monoclonal Anti-3-nitrotyrosine Antibody},
journal = {Revista de Chimie},
volume = {71},
number = {1},
pages = {259-265},
year = {2020},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.20.1.7843},
url = {https://revistadechimie.ro/Articles.asp?ID=7843}
}

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