RAMONA GABRIELA URSU, CATALINA MIHAELA LUCA, ANDREI STEFAN LUCA, ELENA TOADER, LAURENTIU SIMION, LUMINITA SMARANDA IANCU<br>Laboratory Diagnosis for Optimize Therapy of B Hepatitis Virus Infection by Using Biochemical and Molecular Biology Methods

RAMONA GABRIELA URSU, CATALINA MIHAELA LUCA, ANDREI STEFAN LUCA, ELENA TOADER, LAURENTIU SIMION, LUMINITA SMARANDA IANCU
Laboratory Diagnosis for Optimize Therapy of B Hepatitis Virus Infection by Using Biochemical and Molecular Biology Methods

Abstract:

The optimal management of chronic hepatitis B virus (HBV) infections is known to be related with the genotype of HBV in order to prescribe the most appropriate treatment. In this study, including 19 chronic HBV patients from St. Parascheva Clinical Infectious Disease Hospital, Iaşi, we attempted to double test their samples for DNA/HBV detection and genotyping. DNA/HBV evidenced from19 patients known as being chronic infected by HBV were tested by two molecular techniques: Real Time PCR (Polymerase Chain Reaction) and genotyping by hybridization on strips. We obtained identical results from both methods. The most frequent genotype was D (47.4%), followed by genotype A (10,5%); 9 samples were negative, probably because of virus suppressed replication under treatment. The double testing is accurate, time and cost efficient, and for these reasons we propose this double testing like a possible algorithm for routine diagnostic, being time-efficient and accurate for HBV chronic infected patients’ management cases. Keywords: HBV, PCR classic, Real Time PCR, accurate detection

Issue: 2016, Volume 67, Issue 12

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