Gabriela Isopencu, Alina Tanase, AnaMaria Josceanu, Vasile Lavric1<br>Influence of the Operating Parameters on the <br>E. Coli BL21 (DE3) Growth in Fedbatch Bioreactor

Gabriela Isopencu, Alina Tanase, AnaMaria Josceanu, Vasile Lavric1
Influence of the Operating Parameters on the
E. Coli BL21 (DE3) Growth in Fedbatch Bioreactor

Abstract:

The interdependences between environment changes and cellular responses to external stimuli are still not fully understood. Accordingly, in many cases the influence of the individual cellular heterogeneity on the productivity of some metabolites at industrial scale cannot be justified. This study intends to highlight the response of a bacterial population to the modification of certain external stimuli (operating parameters) during the fed-batch cultivation of an E. coli strain. The purpose is to understand how the population of E. coli responds, thus paving the way to a better production of secondary metabolites, such as recombinant proteins. The envisaged operating parameters were temperature and rotational speed of the stirrer, while the response parameters considered were the pH, pO2 and the optical density (OD) of the growing medium. At the same time, the cellular apoptosis, responsible for the reduction of living cells’ concentration, was determined measuring the cells viability by flow cytometry on samples taken at given intervals. The population tends to synchronize in all periods of growth, but more in the stationary phase. Keywords: fed - batch, external stimuli, viability, optical density, flow cytometry, E. coli

Issue: 2014, Volume 65, Issue 12

Pages:

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