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https://doi.org/10.37358/Rev.Chim.1949

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Revista de Chimie (Rev. Chim.), Year 2011, Volume 62, Issue 5,





CATALINA GABRIELA GHEORGHE, OCTAV PANTEA, VASILE MATEI, DORIN BOMBOS, ANCA-FLORENTINA BORCEA
Research on the Influence of Diesel Oil on the Bacterial Stems of Bacillus Subtilis and Pseudomonas Aeruginosa

Abstract:

In this experiment we tested separately the evolution of turbidity for two pure bacterial suspensions, Bacillus subtilis ATCC 2589 and Pseudomonas aeruginosa ATCC 15442, in a concentration of 1 Mc Farland. In comparison with the evolution of pure suspensions we have analyzed the evolution of suspension turbidities in contact with diesel fuel in different concentrations: 100mL Diesel, 200mL Diesel and 300mL Diesel. The samples were incubated at 30-37 degrees Celsius for 72 h ; after 6 h, 24, 48 and 72 h thermostating time, bacterial suspension was photo metered at a wave with 530 nm length for measuring the suspension turbidity. In order to determine the viability of the microorganisms tested in the presence of the analyzed contaminators were made counts of bacterial colonies grown on nutrient agar culture medium (CFU / mL). Keywords: Diesel oil, Bacillus subtilis, Pseudomonas aeruginosa, turbidity


Issue: 2011, Volume 62, Issue 5
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