Alina GHARIBEH BRANIC, Carmen-Manuela PLESA, Nicoleta Gabriela HADARUGA, Aurel ARDELEAN, Daniel Ioan HĂDĂRUGĂ, Valentin LaurenTiu ORDODI, Alexandra Teodora GRUIA5, Alfa Xenia LUPEA<br>A Comparative Study of Juniperus communis and Juniperus virginiana Extracts<br>The Influence of method, solvent, and provenience

Alina GHARIBEH BRANIC, Carmen-Manuela PLESA, Nicoleta Gabriela HADARUGA, Aurel ARDELEAN, Daniel Ioan HĂDĂRUGĂ, Valentin LaurenTiu ORDODI, Alexandra Teodora GRUIA5, Alfa Xenia LUPEA
A Comparative Study of Juniperus communis and Juniperus virginiana Extracts
The Influence of method, solvent, and provenience

Abstract:

The paper presents a comparative study of the Juniperus communis and Juniperus virginiana hydrophobic extracts from the method, solvent, and source influence point of view. Hydrophobic solvents (such as hexane and ethyl acetate) were used for obtaining of J. communis and J. virginiana extracts. Two extraction methods (solvent reflux and sonication) were used for obtaining hydrophobic extracts by using different plant parts (branches, needles, and berries) collected from autochthonous area and other zones from Austria and Syria. Fifteen main compounds were identified and quantified in all hydrophobic extracts by using gas chromatography-mass spectrometry analysis (such as a- and b-pinene, b-phellandrene, caryophyllene, and b-cubebene). The highest amount of b-pinene is identified in the Syrian J. communis branches hexane extract, while b-phellandrene is identified in higher content in the J. virginiana ethyl acetate extract from “Macea” Botanical Garden (Arad, Romania). Principal component analysis of the gas chromatographic data (relative concentration of the main volatile compounds) revealed that the Juniperus species can be classified according to the mono- and sesquiterpene concentrations (limonene,a-pinene, humulene, caryophyllene, cubebene); the provenience of these samples can also be classified by this procedure, but the biologically active compounds concentrations had no significance on the classification according to the extraction method and solvent type. Keywords: Juniperus communis, Juniperus virginiana, sonication extraction, refluxing extraction, principal component analysis

Issue: 2011, Volume 62, Issue 5

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This article is published under the Creative Commons Attribution 4.0 International License

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