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Revista de Chimie (Rev. Chim.), Year 2008, Volume 59, Issue 8,

https://doi.org/10.37358/RC.08.8.1903

Marcela Elisabeta Barbinta-Patrascu, Nicoleta Badea, Laura Georgeta Tugulea, Maria Giurginca, Aurelia Meghea

Oxidative Stress Simulation on Chlorophyll a Lipid Model Membranes - Chemiluminescent Studies

Abstract:

Oxidative stress was simulated on small unilamellar liposomes with chlorophyll a as model membranes. Chlorophyll a incorporated in the lipid bilayer of liposomes was used as a spectral marker to monitor the oxidative damage on liposomes. Small unilamellar vesicles were obtained by the thin-film hydration method using the lipids: b-stearoyl-g-oleoyl L-a-phosphatidylcholine and dimyristoyl phosphatidylcholine. The chemiluminescence assay was used to quantify the oxidative stress in model membranes. The antioxidative effect caused by vitamin E inserted into lipid bilayer of liposomes has been also monitored. Different lipid membranes were tested. The chemiluminiscence studies indicate that the liposomes system with a molar ratio Chla: a-toc: lipid = 1:1:100 exhibit a great increase of antioxidant activity.

Keywords:
small unilamellar liposomes; lipid model membranes; chlorophyll a; vitamin E; oxidative stress; chemiluminescence

Issue: 2008, Volume 59, Issue 8
Pages:
Publication date: 2008/9/9
https://doi.org/10.37358/RC.08.8.1903
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