In silico Analysis of 1,4-butanediol Heterologous Pathway Impact on Escherichia coli  Metabolism

Bodor, Zsolt; Tompos, Lehel; Nechifor, Aurelia Cristina; Bodor, Katalin

Zsolt Bodor, Lehel Tompos, Aurelia Cristina Nechifor, Katalin Bodor

In silico Analysis of 1,4-butanediol Heterologous Pathway Impact on Escherichia coli Metabolism

Abstract:

The bio-based synthesis of 1,4-butanediol (BDO), a key compound in many industries, has recently been achieved in Escherichia coli, however the yield even in glucose was far below the theoretical maximum. Furthermore, the impact of the BDO pathway on cell metabolism is yet to be discovered. The main objective of this study was to in silico improve and analyze the production potential of BDO on glucose and glycerol and evaluate the interaction between native and non-native pathways for wild-type and mutant strains using a simple biosynthetic pathway. The maximum production potential and changes in metabolic fluxes were simulated by different objective functions (biomass and BDO) and the reactions with highest differences were identified under different environmental conditions. Considering the outcomes 80% of the reactions with significant flux change were identical for all conditions simulated. Flux variability analysis was carried out to decipher the variation of fluxes and flux span changes (SC) were calculated. To further analyse the reactions with SC over 1 mmol gDW-1h-1 and to calculate the correlation coefficients for WT and mutant strains uniform random sampling was carried out. Most important variations in correlation patterns were observed for reactions in the mutant model. On the other hand, the addition, elimination and optimization of different pathways significantly affected the pairwise correlation patterns as well as the entire solution space of the network.

Keywords:
1;4-butanediol; E. coli; flux variability analysis; random sampling; in silico; COBRA

Issue: 2019, Volume 70, Issue 10

Pages: 3448-3455

Publication date: 2019/11/15

https://doi.org/10.37358/RC.19.10.7574

Download Pdf Article

This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
BODOR, Z., TOMPOS, L., NECHIFOR, A.C., BODOR, K., In silico Analysis of 1,4-butanediol Heterologous Pathway Impact on Escherichia coli Metabolism, Rev. Chim., 70(10), 2019, 3448-3455.

Vancouver
Bodor Z, Tompos L, Nechifor AC, Bodor K. In silico Analysis of 1,4-butanediol Heterologous Pathway Impact on Escherichia coli Metabolism. Rev. Chim.[internet]. 2019 Oct;70(10):3448-3455. Available from: https://doi.org/10.37358/RC.19.10.7574

APA 6th edition
Bodor, Z., Tompos, L., Nechifor, A.C. & Bodor, K. (2019). In silico Analysis of 1,4-butanediol Heterologous Pathway Impact on Escherichia coli Metabolism. Revista de Chimie, 70(10), 3448-3455. https://doi.org/10.37358/RC.19.10.7574

Harvard
Bodor, Z., Tompos, L., Nechifor, A.C., Bodor, K. (2019). 'In silico Analysis of 1,4-butanediol Heterologous Pathway Impact on Escherichia coli Metabolism', Revista de Chimie, 70(10), pp. 3448-3455. https://doi.org/10.37358/RC.19.10.7574

IEEE
Z. Bodor, L. Tompos, A.C. Nechifor, K. Bodor, "In silico Analysis of 1,4-butanediol Heterologous Pathway Impact on Escherichia coli Metabolism". Revista de Chimie, vol. 70, no. 10, pp. 3448-3455, 2019. [online]. https://doi.org/10.37358/RC.19.10.7574

Text
Zsolt Bodor, Lehel Tompos, Aurelia Cristina Nechifor, Katalin Bodor,
In silico Analysis of 1,4-butanediol Heterologous Pathway Impact on Escherichia coli Metabolism,
Revista de Chimie,
Volume 70, Issue 10,
2019,
Pages 3448-3455,
ISSN 2668-8212,
https://doi.org/10.37358/RC.19.10.7574.
(https://revistadechimie.ro/Articles.asp?ID=7574)
Keywords: 1;4-butanediol; E. coli; flux variability analysis; random sampling; in silico; COBRA

RIS
TY - JOUR
T1 - In silico Analysis of 1,4-butanediol Heterologous Pathway Impact on Escherichia coli Metabolism
A1 - Bodor, Zsolt
A2 - Tompos, Lehel
A3 - Nechifor, Aurelia Cristina
A4 - Bodor, Katalin
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2019
VL - 70
IS - 10
SP - 3448
EP - 3455
UR - https://doi.org/10.37358/RC.19.10.7574
KW - 1
KW - 4-butanediol
KW - E. coli
KW - flux variability analysis
KW - random sampling
KW - in silico
KW - COBRA
ER -

BibTex
@article{RevCh2019P3448,
author = {Bodor Zsolt and Tompos Lehel and Nechifor Aurelia Cristina and Bodor Katalin},
title = {In silico Analysis of 1,4-butanediol Heterologous Pathway Impact on Escherichia coli Metabolism},
journal = {Revista de Chimie},
volume = {70},
number = {10},
pages = {3448-3455},
year = {2019},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.19.10.7574},
url = {https://revistadechimie.ro/Articles.asp?ID=7574}
}

<<<< back

PUBLISHING SECTION

Author Guidelines

* Paper Drafting

* Paper Template

* Ethical Statement

* Paper Submission

Ghidul Autorului (ro)

Open Access & Archiving Policy

Search Authors

LATEST NEWS
	NUMAR OMAGIAL PETRU PONI.. detalii
	Revista de Chimie - 70 de ani de aparitie neintrerupta.. detalii
	English-Romanian Dictionary for Mechanical Engineering (download pdf)