HPLC Screening of Bioactives Compounds and Antioxidant Capacity of Different Hypericum Species

Gitea, Daniela; Vicas, Simona; Gitea, Manuel Alexandru; Nemeth, Sebastian; Tit, Delia Mirela; Pasca, Bianca; Purza, Lavinia; Iovan, Ciprian

Daniela Gitea, Simona Vicas, Manuel Alexandru Gitea, Sebastian Nemeth, Delia Mirela Tit, Bianca Pasca, Lavinia Purza, Ciprian Iovan

HPLC Screening of Bioactives Compounds and Antioxidant Capacity of Different Hypericum Species

Abstract:

Our study compares the content in polyphenolic compounds and hypericin, in four species of Hypericum - H. perforatum L., H. maculatum Cr., H. hirsutum L., H. tetrapterum Fr. (syn. Hypericumacutum Mnch.) harvested from spontaneous flora in the north-western area of Transylvania, Romania. These species represent an important source of such compounds with different biological actions. After making the extracts, they were subjected to HPLC-SM analysis. The presence of rutoside in the largest amount (462.82 mg %) in the H. perforatum extract was observed, this containing most of the flavonoid heterosides. For the species H. maculatum, the presence in a much higher amount of the hyperoside (976.36 mg %) is characteristic compared to the other species. Quercetol is the best represented of the flavonoid aglycons, its concentration being the highest in H. hirsutum (659.66 mg %). The hypericin content ranges from 0.2171 g % in the H. tetrapterum extract, to 0.0314 g % in the methanol extract of H. maculatum.The highest antioxidant properties measured by FRAP method were recorded in the case of H. perforatum and H. maculatum.

Keywords:
Hypericum sp.; antioxidant activity; polyphenolic compounds; hypericin; HPLC-MS

Issue: 2018, Volume 69, Issue 2

Pages: 305-309

Publication date: 2018/3/15

https://doi.org/10.37358/RC.18.2.6095

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This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
GITEA, D., VICAS, S., GITEA, M.A., NEMETH, S., TIT, D.M., PASCA, B., PURZA, L., IOVAN, C., HPLC Screening of Bioactives Compounds and Antioxidant Capacity of Different Hypericum Species, Rev. Chim., 69(2), 2018, 305-309.

Vancouver
Gitea D, Vicas S, Gitea MA, Nemeth S, Tit DM, Pasca B, Purza L, Iovan C. HPLC Screening of Bioactives Compounds and Antioxidant Capacity of Different Hypericum Species. Rev. Chim.[internet]. 2018 Feb;69(2):305-309. Available from: https://doi.org/10.37358/RC.18.2.6095

APA 6th edition
Gitea, D., Vicas, S., Gitea, M.A., Nemeth, S., Tit, D.M., Pasca, B., Purza, L. & Iovan, C. (2018). HPLC Screening of Bioactives Compounds and Antioxidant Capacity of Different Hypericum Species. Revista de Chimie, 69(2), 305-309. https://doi.org/10.37358/RC.18.2.6095

Harvard
Gitea, D., Vicas, S., Gitea, M.A., Nemeth, S., Tit, D.M., Pasca, B., Purza, L., Iovan, C. (2018). 'HPLC Screening of Bioactives Compounds and Antioxidant Capacity of Different Hypericum Species', Revista de Chimie, 69(2), pp. 305-309. https://doi.org/10.37358/RC.18.2.6095

IEEE
D. Gitea, S. Vicas, M.A. Gitea, S. Nemeth, D.M. Tit, B. Pasca, L. Purza, C. Iovan, "HPLC Screening of Bioactives Compounds and Antioxidant Capacity of Different Hypericum Species". Revista de Chimie, vol. 69, no. 2, pp. 305-309, 2018. [online]. https://doi.org/10.37358/RC.18.2.6095

Text
Daniela Gitea, Simona Vicas, Manuel Alexandru Gitea, Sebastian Nemeth, Delia Mirela Tit, Bianca Pasca, Lavinia Purza, Ciprian Iovan,
HPLC Screening of Bioactives Compounds and Antioxidant Capacity of Different Hypericum Species,
Revista de Chimie,
Volume 69, Issue 2,
2018,
Pages 305-309,
ISSN 2668-8212,
https://doi.org/10.37358/RC.18.2.6095.
(https://revistadechimie.ro/Articles.asp?ID=6095)
Keywords: Hypericum sp.; antioxidant activity; polyphenolic compounds; hypericin; HPLC-MS

RIS
TY - JOUR
T1 - HPLC Screening of Bioactives Compounds and Antioxidant Capacity of Different Hypericum Species
A1 - Gitea, Daniela
A2 - Vicas, Simona
A3 - Gitea, Manuel Alexandru
A4 - Nemeth, Sebastian
A5 - Tit, Delia Mirela
A6 - Pasca, Bianca
A7 - Purza, Lavinia
A8 - Iovan, Ciprian
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2018
VL - 69
IS - 2
SP - 305
EP - 309
UR - https://doi.org/10.37358/RC.18.2.6095
KW - Hypericum sp.
KW - antioxidant activity
KW - polyphenolic compounds
KW - hypericin
KW - HPLC-MS
ER -

BibTex
@article{RevCh2018P305,
author = {Gitea Daniela and Vicas Simona and Gitea Manuel Alexandru and Nemeth Sebastian and Tit Delia Mirela and Pasca Bianca and Purza Lavinia and Iovan Ciprian},
title = {HPLC Screening of Bioactives Compounds and Antioxidant Capacity of Different Hypericum Species},
journal = {Revista de Chimie},
volume = {69},
number = {2},
pages = {305-309},
year = {2018},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.18.2.6095},
url = {https://revistadechimie.ro/Articles.asp?ID=6095}
}

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