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https://doi.org/10.37358/Rev.Chim.1949

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Revista de Chimie (Rev. Chim.), Year 2017, Volume 68, Issue 5, 997-1001

https://doi.org/10.37358/RC.17.5.5597

Mihaela Magdalena Mitache, Irina Gheorghe, Georgeta Totea, Coralia Bleotu, Carmen Curutiu, Daniel Cochior, Elena Rusu, Mariana Carmen Chifiriuc

Biochemical, Virulence and Resistance Features in Bacterial Strains Recovered from Hospital Surfaces after Decontamination with Quaternary Ammonium Compounds, Triclosan and Iodine Desinfectatnts

Abstract:

The purpose of this study was to investigate the resistance and virulence markers in microbial strains isolated from the hospital environment, recovered after surfaces decontamination with quaternary ammonium compounds, triclosan, iodine disinfectants, in order to predict their role in healthcare-associated infections. The resistance phenotypes were established using disk diffusion and double-disk diffusion test. Simplex and multiplex PCR assays were used to identify the genetic support of cell-associated and soluble virulence factors The Gram-negative bacterial strains isolated from hospital surfaces after decontamination were resistant in high proportions to beta-lactam antibiotics, including penicillins and associations with beta-lactamase inhibitors, third and fourth generation cephalosporins and carbapenems (encoded by blaTEM, blaNDMlike blaCTX-Mlike, blaOXA-48like genes), quinolones (QnrA, gyrB, parE genes), aminoglycosides (aac3Ia gene), and tetracyclines. Regarding the virulence profiles, P. aeruginosa strains revealed the plcH gene (86.6%) followed by plcH, ExoS, algD and ExoU, while the Enterobacteriaceae strains, fimH (24%), followed by papC, sfaDE, hlyA, cnf1, eaea and VT2 genes. S. aureus isolates revealed the SCCmec cassette of type II (40%), followed by type V, IIIA and type IV (2B) and the clfA, clfB, and can adhesion genes. These findings demonstrate that incorrect practices in biocides use, regarding contact time and concentrations or instruments and surfaces insufficient cleaning before disinfection can decrease their effect and favor the persistence of resistant and virulent microbial strains in the hospital environment. The multiple drug resistance and virulence determinants, encoded by diverse genetic elements suggest the potential of these strains to persist and initiate hospital-associated infections.
Keywords:
resistance; virulence; biocides

Issue: 2017, Volume 68, Issue 5
Pages: 997-1001
Publication date: 2017/6/15
https://doi.org/10.37358/RC.17.5.5597
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Creative Commons License
This article is published under the Creative Commons Attribution 4.0 International License
Citation Styles
Cite this article as:
MITACHE, M.M., GHEORGHE, I., TOTEA, G., BLEOTU, C., CURUTIU, C., COCHIOR, D., RUSU, E., CHIFIRIUC, M.C., Biochemical, Virulence and Resistance Features in Bacterial Strains Recovered from Hospital Surfaces after Decontamination with Quaternary Ammonium Compounds, Triclosan and Iodine Desinfectatnts, Rev. Chim., 68(5), 2017, 997-1001.

Vancouver
Mitache MM, Gheorghe I, Totea G, Bleotu C, Curutiu C, Cochior D, Rusu E, Chifiriuc MC. Biochemical, Virulence and Resistance Features in Bacterial Strains Recovered from Hospital Surfaces after Decontamination with Quaternary Ammonium Compounds, Triclosan and Iodine Desinfectatnts. Rev. Chim.[internet]. 2017 May;68(5):997-1001. Available from: https://doi.org/10.37358/RC.17.5.5597


APA 6th edition
Mitache, M.M., Gheorghe, I., Totea, G., Bleotu, C., Curutiu, C., Cochior, D., Rusu, E. & Chifiriuc, M.C. (2017). Biochemical, Virulence and Resistance Features in Bacterial Strains Recovered from Hospital Surfaces after Decontamination with Quaternary Ammonium Compounds, Triclosan and Iodine Desinfectatnts. Revista de Chimie, 68(5), 997-1001. https://doi.org/10.37358/RC.17.5.5597


Harvard
Mitache, M.M., Gheorghe, I., Totea, G., Bleotu, C., Curutiu, C., Cochior, D., Rusu, E., Chifiriuc, M.C. (2017). 'Biochemical, Virulence and Resistance Features in Bacterial Strains Recovered from Hospital Surfaces after Decontamination with Quaternary Ammonium Compounds, Triclosan and Iodine Desinfectatnts', Revista de Chimie, 68(5), pp. 997-1001. https://doi.org/10.37358/RC.17.5.5597


IEEE
M.M. Mitache, I. Gheorghe, G. Totea, C. Bleotu, C. Curutiu, D. Cochior, E. Rusu, M.C. Chifiriuc, "Biochemical, Virulence and Resistance Features in Bacterial Strains Recovered from Hospital Surfaces after Decontamination with Quaternary Ammonium Compounds, Triclosan and Iodine Desinfectatnts". Revista de Chimie, vol. 68, no. 5, pp. 997-1001, 2017. [online]. https://doi.org/10.37358/RC.17.5.5597


Text
Mihaela Magdalena Mitache, Irina Gheorghe, Georgeta Totea, Coralia Bleotu, Carmen Curutiu, Daniel Cochior, Elena Rusu, Mariana Carmen Chifiriuc,
Biochemical, Virulence and Resistance Features in Bacterial Strains Recovered from Hospital Surfaces after Decontamination with Quaternary Ammonium Compounds, Triclosan and Iodine Desinfectatnts,
Revista de Chimie,
Volume 68, Issue 5,
2017,
Pages 997-1001,
ISSN 2668-8212,
https://doi.org/10.37358/RC.17.5.5597.
(https://revistadechimie.ro/Articles.asp?ID=5597)
Keywords: resistance; virulence; biocides


RIS
TY - JOUR
T1 - Biochemical, Virulence and Resistance Features in Bacterial Strains Recovered from Hospital Surfaces after Decontamination with Quaternary Ammonium Compounds, Triclosan and Iodine Desinfectatnts
A1 - Mitache, Mihaela Magdalena
A2 - Gheorghe, Irina
A3 - Totea, Georgeta
A4 - Bleotu, Coralia
A5 - Curutiu, Carmen
A6 - Cochior, Daniel
A7 - Rusu, Elena
A8 - Chifiriuc, Mariana Carmen
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2017
VL - 68
IS - 5
SP - 997
EP - 1001
UR - https://doi.org/10.37358/RC.17.5.5597
KW - resistance
KW - virulence
KW - biocides
ER -


BibTex
@article{RevCh2017P997,
author = {Mitache Mihaela Magdalena and Gheorghe Irina and Totea Georgeta and Bleotu Coralia and Curutiu Carmen and Cochior Daniel and Rusu Elena and Chifiriuc Mariana Carmen},
title = {Biochemical, Virulence and Resistance Features in Bacterial Strains Recovered from Hospital Surfaces after Decontamination with Quaternary Ammonium Compounds, Triclosan and Iodine Desinfectatnts},
journal = {Revista de Chimie},
volume = {68},
number = {5},
pages = {997-1001},
year = {2017},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.17.5.5597},
url = {https://revistadechimie.ro/Articles.asp?ID=5597}
}
 
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