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Revista de Chimie (Rev. Chim.), Year 2011, Volume 62, Issue 12,





Alina Nicolescu, Brion Dolenko, Tedros Bezabeh, Lorena-Ivona Stefan, Coziana Ciurtin,Eugenia Kovacs, Ian C. P. Smith, Bogdan C. Simionescu, Calin Deleanu
Diagnosis of Type II Diabetes based on Non-glucose Regions
of 1H NMR Spectra of Urine A metabonomic approach

Abstract:

A NMR dataset with non-buffered urine samples consisting of 73 controls and 94 type II diabetes was subject to an in-house statistical classifier. A model was developed based on two glucose-free regions of the spectrum and those maximally discriminatory subregions selected most often by the algorithm were noted. The final classifier achieved 83.0% sensitivity and 83.6% specificity, with 83.2% overall accuracy. There were five spectral subregions selected by the algorithm as most relevant for discrimination. The protocol works well with non-buffered samples and has the potential for an automated clinical diagnosis of diabetes. Keywords: NMR spectroscopy, urinary metabolites, diabetes, statistical classification, chemometry


Issue: 2011, Volume 62, Issue 12
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